Comparison of Different Elisa Reagent Kits for Diagnosis of Tomato Brown Rugose Fruit Virus in Tomato Plant
Özet
This study evaluates four commercial Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) kits—from AGDIA, BIOREBA AG, LOEWE Biochemica, and Leibniz Institute DSMZ—for the diagnosis of Tomato Brown Rugose Fruit Virus (ToBRFV) in tomato plants (Solanum lycopersicum cv. Moneymaker). ToBRFV is an emerging, highly virulent tobamovirus that poses a severe threat to global tomato production, capable of infecting resistant cultivars harboring the Tm22 gene. The virus spreads easily through infected seeds, plant debris, direct plant-to-plant mechanical contact, contaminated clothing, water solutions, and bumble bees during greenhouse pollination. To evaluate diagnostic accuracy, specificity, and cross-reactivity, laboratory assays included positive reference controls for related viruses: Pepper mild mottle virus (PMMoV), Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), Cucumber green mottle mosaic virus (CGMMV), and a general Tobamovirus control. Results across three experimental repetitions demonstrated that the AGDIA DAS-ELISA kit produced high absorbance values for ToBRFV-infected samples without showing any cross-reactivity toward the other tobamoviruses tested. In contrast, the kits manufactured by BIOREBA AG, LOEWE Biochemica, and DSMZ showed distinct cross-reactivity with ToMV and Tobamovirus controls, yielding elevated absorbance readings close to ToBRFV levels. Therefore, implementing rapid, reliable, and highly specific diagnostic kits like AGDIA is vital to detect ToBRFV early, restrict viral dissemination, and execute timely eradication protocols in tomato cultivation.
Referanslar
Adams, M.J., Antoniw, J.F., Kreuze, J., 2009. Virgaviridae: a new family of rod-shaped plant viruses. Arch Virol 154:1967–1972.
Alkowni, R., Alabdallah, O., and Fadda, Z., 2019. Molecular identification of tomato brown rugose fruit virus in tomato in Palestine. Journal of Plant Pathology, Volume 101, Issue 3, pp 719–723.
Bernabé-Orts, J.M., Torre, C., Méndez-López, E., Hernando, Y., Aranda, M.A., 2021. New Resources for the Specific and Sensitive Detection of the Emer¬ging Tomato Brown Rugose Fruit Virus. Viruses, 13, 1680.
Broadbent, L., 1976. Epidemiology and control of tomato mosaic virus. Annu Rev Phytopathol 14:75–96Hanssen IM, Lapidot M, Thomma BPHJ, 2010. Emerging viral diseases of tomato crops. Mol Plant Microbe Interact 23:539–548.
Cambrón-Crisantos, J.M., Rodríguez-Mendoza, J., Valencia-Luna, J.B., Alcasi¬o-Rangel, S., García-Ávila, C.J., López-Buenfil, J.A., and Ochoa-Martínez, D.L., 2018. First report of Tomato brown rugose fruit virus (ToBRFV) in Michoacan, Mexico. Mexican Journal of Phytopathology, 37(1): 185-192.
Chitambar, J., 2018. California pest rating for Tomato brown rugose fruit virus. Pest Rating:A, 1-8 https://blogs.cdfa.ca.gov/Section3162/?p=5843
Clark, M.F., Adams, A.N., 1977. Characteristics of the microplate method of enzyme linked immunosorbent assay for dedection of plant viruses J. of Gen. Virol. 34:475-483.
Celik, N., Özalp, R., Celik, I., 2010. Bazı Biber Hat ve Çeşitlerinin Tobacco mo¬saic tobamovirus (TMV)’E Dayanıklılığının Mekanik İnokulasyon ve Elısa Testleri İle Belirlenmesi. Batı Akdeniz Tarımsal Araştırma Enstitüsü Derim Dergisi, 27(2):1-9.
Deom, C. M., Quan, S. and He, X.Z., 1997. Replicase proteins as determinants of phloem-dependent long-distance movement of tobamoviruses in tobacco. Protoplasma, 199:1-8
Dombrovsky, A., Smith, E., 2017. Seed Transmission of Tobamoviruses: Aspe¬cts of Global Disease Distribution. pp: 234-260. In: Jose C. Jimenez-Lopez (ed.). Seed Bio-logy. IntechOpen. 233-260p. http://doi.org/10.5772/inte¬cho-pen.70244.
FAO, 2020. Food and Agriculture Organization of the United Nations -FAO. https://www.fao.org/faostat/en/#data/QCL/visualize.
Fidan, H., Sarikaya, P., Calis, O., 2019. First report of Tomato brown rugose fruit virus on tomato in Turkey. New Disease Reports 39, 18.
Hanssen, Im., Lapidot, M., Thomma, BP., 2010. Emerging viral diseases of toma¬to crops. Mol Plant Microbe Interact. 23(5):539±48. doi: 10.1094/MPMI- 23-5-0539 PMID: 20367462.
Luria, N., Smith, E., Reingold, V., Bekelman, I., Lapidot, M., Levin, I., Elad, N., Tam, Y., Sela, Abu-Ras, A., Ezra, N., Haberman, A., Yitzhak, L., Lachman, O., Dombrovsky, A., 2017. A new Israeli Tobamovirus isolate infects toma¬to plants harboring Tm-22 resistance genes. PLoS ONE 12 (1):e0170429. doi:10.1371/journal.pone.0170429:p1-19.
Menzel, W., Knierim, D., Winter, S., Hamacher, J., Heupel, M., 2019. First report of Tomato brown rugose fruit virus infecting tomato in Germany. New Di¬sease Reports 39, 1.
Pagan, I., Firth, C., Holmes, Ec., 2010. Phylogenetic analysis reveals rapid evo¬lutionary dynamics in the plant RNA virus genus Tobamovirus. J Mol Evol 71:298–307.
Panno, S., Caruso, A. G., Davino, S., 2019. First Report of Tomato Brown Rugo¬se Fruit Virus on Tomato Crops in Italy. The American Phytopathological Society (APS), https://doi.org/10.1094/PDIS-12-18-2254-PDN
Salem, N., Mansou, R A., Ciuffo, M., Falk, B. W., Turina, M., 2016. A new tobamovirus infecting tomato crops in Jordan. Archives of Virology, 161(2):503–506.
Skelton, A., Buxton-Kirk, A., Ward R., Harju, V., Frew, L., Fowkes, A., Long, M., Negus, A., Forde, S., Adams, I.P., Pufal H, Mcgreig S., Weekes R. and Fox A., 2019. First report of Tomato brown rugose fruit virus in tomato in the United Kingdom, New Disease Reports 40, 12. [http://dx.doi.org/10.5197 /j.2044-0588.2019.040.012]
Yan, Z-Y., Ma, H-Y., Han, S-L., Geng, C., Tian, Y-P., Li, X-D., 2019. First report of Tomato brown rugose fruit virus infecting tomato in China. Plant Dise¬ase (early view). DOI: 10.1094/PDIS-05-19-1045-PDN.
